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Definition of Southern blot
1. Noun. (biochemistry) A sheet to which DNA has been transfered from electrophoresis gel, used for detection of a specific DNA sequence in DNA samples. ¹
2. Verb. (transitive biochemistry) To use a Southern blot on (some DNA). ¹
¹ Source: wiktionary.com
Medical Definition of Southern blot
1. A technique used for searching for a specific DNA fragment. The process is as follows: 1. separate DNA fragments by gel electrophoresis 2. Change pH of gel to basic, thus allowing disruption of H-bonds 3. blot gel with nitrocellulose paper 4. Heat paper so as to fix DNA fragments 5. probe with labelled messenger RNA or cDNA 6. wash Complementary mRNA/cDNA fragments will have hybridised. (09 Oct 1997)
Lexicographical Neighbors of Southern Blot
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